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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Liver Int. 2013 Apr 1;33(7):1044–1055. doi: 10.1111/liv.12164

Figure 3. Primary hepatocytes are resistant to gliotoxin toxicity in vitro.

Figure 3

(A) MTT assay on primary rat hepatocytes, primary rat HSCs, mixed cultures of rat hepatocytes and NPCs, human microvascular endothelial cells, and HSC-T6 cells treated with gliotoxin for 4 hours. (B) Thymidine incorporation assay on primary rat hepatocytes treated with a low dose of gliotoxin for 4 consecutive days in culture. (C) TUNEL staining on primary rat hepatocytes, HSCs, and mixed cultures (hepatocytes and NPCs) treated with gliotoxin for 4 hours.