Figure 2. Morphology and expansion capacity of hASCs cultured in 6%HS or 10% FBS.

(A) Images of hASCs cultures at days 1 and 4 after seeding (200 cells/cm2). Cells were obtained from the same donor and cultured with 6% HS or 10% FBS, respectively (n = 3 donors). All the images were captured at 10X magnification in an Olympus microscope. (B) Proliferation kinetics growth curve of hASCs in HS and FBS from seeding until day 11. (C) Mean cumulative population doublings for HS (black) and FBS (white). Cells used for both conditions were obtained from the same 3 donors (n = 3) and cultured until passage 18.