Table 1. Up-regulated genes between passages involved in significant KEEG pathways.

	Number ofgenes BH<0.05	KEGG Pathway	Genes in KEGG pathway	Fisher exactp-value
Passage 1 versus Isolation	63	ECM-receptor interaction	COL1A2, FN1,SDC1	2.5·10−3
		Biosynthesis of unsaturatedfatty acids	ELOVL6, FADS1	2.3·10−3
Passage 3 versus passage 1	111	Piruvate metabolism	ACAT2, ALDH1B1, PDHB	1.3·10−3
		Butanoate metabolism	E2F5,SMAD2,ANAPC1,CDC16,CDNK2A, ORC4L,TFDP2	1.0·10−4
		Cell cycle	ACAT2, ALDH1B1, GLO1, PDHB	1.1·10−4
Passage 5 versus passage 3	209	Cell division pathway	BBC3, CHEK1, FAS, THBS1	7.2·10−3
		Ribosome	MRPL13, RPL22L1, RPS10, RPLP0	1.7·10−2
		Pyruvate metabolism	ACAT2, ALDH7A1, ME1	1.0·10−2
		Regulation of actin cytoskeleton	WASF1, CFL2, FGF2, PFN2, RRAS2	3.5·10−2