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. Author manuscript; available in PMC: 2014 Feb 27.
Published in final edited form as: Neurobiol Dis. 2012 Aug 27;0:13–21. doi: 10.1016/j.nbd.2012.08.016

Figure 5.

Figure 5

HIF-PHD inhibitors prevent N2A cell proliferation. A-B, N2A cells were plated in 96-well plates, treated with HIF-PHD inhibitors, and then assayed for viability by calcein labeling and cell counting with a flash cytometer. A, N2A cells proliferate over 3 days (top) to cover the well surface, whereas proliferation is inhibited in DFO-treated cells (bottom). B, cell counts from cultures treated with DFO 50 μM, CPO 1 μM, DHB 20 μM, or DMOG 250 μM show that HIF-PHD inhibition impeded N2A cell proliferation compared untreated cells. Scale bar = 1 mm. Data are normalized to cell counts from control wells on day 1. Cell counts from PHD inhibitor-treated cells are significantly lower than control at days 2 and 3, *=P<0.01, two-way ANOVA-Bonferroni’s post test, n=8.