Figure 4. Establishment of the HepG2-PXR stable cell line.

The selection methods of HepG2-PXR and HepG2-Vector cells were described in Materials and Methods. A to D. The selected cells were verified for PXR expression using RT-PCR (A), western blot using an anti-PXR antibody (B) and an anti-flag antibody (C), and immunofluorescence using an anti-PXR antibody (D). E. Dual-luciferase assay of transcriptional activity of PXR on CYP3A4 in HepG2-Vector and HepG2-PXR cells. pCYP3A4-Luc and pRL-tk were transfected into these two cell types for 24 h, and then treated with rifampicin (10 µM) for another 24 h. Three independent experiments were performed. *, P<0.05.