Figure 2. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) validation of selected genes.
Relative gene expression fold change from qRT-PCR from retinal microglia from 24-month old mice (3 -6 biological repeats were used for each experiment, with one sample replicate per biological replicate), normalized relative to those from 3-month old mice (A) is compared to the relative fold changes for the same genes predicted from microarray analysis (B). Error bars indicate ± SEM.
