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. Author manuscript; available in PMC: 2014 Mar 13.
Published in final edited form as: Biochemistry. 2013 Feb 27;52(10):10.1021/bi400037w. doi: 10.1021/bi400037w

FIGURE 1.

FIGURE 1

“pH dependence of Mtb ICDH-1.” (A) kcat and kcat/KNADP+ determined at saturating isocitrate and varying NADP+. (A) (●) Log kcat fitting to eq 5 determined pKa = 5.6 ± 0.1. (■) Log kcat/KNADP+ fitting to eq 5 determined pKa = 6.3 ± 0.2. (B) kcat and kcat/Kisocitrate determined at saturating NADP+ and varying isocitrate. (●) Log kcat fitting to eq 5 determined pKa = 5.5 ± 0.2. (■) Log kcat/Kisocitrate fitting to the inverse of eq 5 determined pKa = 5.8 ± 0.1.