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. Author manuscript; available in PMC: 2014 Mar 13.
Published in final edited form as: Biochemistry. 2013 Feb 27;52(10):10.1021/bi400037w. doi: 10.1021/bi400037w

FIGURE 2.

FIGURE 2

“Primary Kinetic Isotope Effects.” (A) Primary KIE at 500 μM NADP+ and varying (20-300 μM) (●) isocitrate or (■) [2R-2H]isocitrate. DV = 1.3 ± 0.1, D[V/K[2R-2H]isocitrate] = 1.5 ± 0.2. (B) pH dependence of (●) DV and (■) D[V/K[2R-2H]isocitrate] at 500 μM NADP+ and varying (5 – 150 μM) isocitrate or [2R-2H]isocitrate.