Table 2.
Rate constants for refolding to the native state of P3-strengthening mutants.
| Ribozyme | 37 °C | 37 °C; 150 mM Added Na+ | 50 °C; 150 mM Added Na+ | |||
|---|---|---|---|---|---|---|
|
| ||||||
| krefold (min−1) | Rel | krefold (min−1) | Rel | krefold (min−1) | Rel | |
| Wild-Type | 0.0023 | (1) | 0.66 | (1) | 33 a | (1) |
| U101A | 0.00035 | 0.15 | 0.19 | 0.29 | -- | -- |
| U273A | -- | -- | 0.024 | 0.036 | -- | -- |
| U273G | -- | -- | 0.015 | 0.023 | -- | -- |
| U101C:U273G | -- | -- | -- | -- | 0.024 | 0.00073 |
a
Calculated as the value measured under standard conditions at 37 °C (0.0023 min−1 column 1) multiplied by the effects of adding 150 mM Na+ (290-fold, column 2) and raising the temperature to 50 °C (50-fold, ref. 33).