Table 4. Quantification of carbonylated proteins and peptides and location of the modification.
| Proteins name | Protein ratio(UV/DARK) | p val prot. | Nb of total identifiedpept. (>95%) | Nb of totalquantified pept.(pval <0.1) | Carbonylated Peptide ratio(UV/DARK) | p val pept. | Sequence of carbonylated peptides |
| VAS14_09599 acetoacetyl-CoA | 0.8 | 0.07 | 81 | 10 | 0.39 | 0.09 | MSKVALVTGAKGGIGSSITQALVDAGFR |
| eductase* | 0.69 | 0.44 | VVATYYQTGEKAAQEWLAANNYSSESVR | ||||
| VAS14_14994 transketolase* | 0.96 | 0.10 | 60 | 5 | 0.26 | 0.19 | TPGHPEYGFAPGVETTTGPLGQGITNAVGMALAEK |
| 0.77 | 0.08 | TPGHPEYGYAPGVETTAGPLGQGITNAVGMALAEK | |||||
| VAS14_04158 arginine ABC | 0.51 | 0.05 | 81 | 11 | 0.75 | 0.78 | FAMEATYAPFEYVDENNQIQGFDVDIAK |
| transporter | 2.56 | 0.06 | FAMEATYAPFERMDENNQIQGFDVDIAK | ||||
| VAS14_04998 elongation factor EF-2 | 1.26 | 0.09 | 151 | 13 | 0.76 | 0.65 | TGEVHDGEATTDFMEQEAER |
| 2.12 | 0.36 | GMQLVLDAVVDYLPSPTEVDPQPLTDPNTGEPTGEVATVSADEPLK | |||||
| 5.21 | 0.07 | AGPQLLEPIMNVDVFTPEDHVGDVIGDLNR | |||||
| VAS14_05968 hypothetical protein* | 0.74 | 0.1 | 46 | 3 | 0.79 | 0.09 | SDVSNNTLTTEPETATVEFTQTSGAIDMSNSK |
| VAS14_06513 formate | 0.49 | 0.08 | 132 | 23 | 0.15 | 0.01 | TPEYDELFSGDPIWATESMGGMGLDGR |
| acetyltransferase* | 0.16 | 0.01 | TPEYDELFSGDPIWATDSMGGMGLDGR | ||||
| 0.31 | 0.01 | TPEYDELFSGDPIWATESMGGMGLDGR | |||||
| VAS14_20236 phosphoglycerate | 0.57 | 0.04 | 75 | 3 | 0.47 | 0.01 | SASDIAADDMVLDLGPDSAQALADIIMNAK |
| kinase* | |||||||
| VAS14_07384 ompL_phopr porin-like | 1.11 | 0.08 | 73 | 15 | 4.53 | 0.02 | ADGALGMLTDVTDIMAYAGSVVGSTK |
| protein L precursor* | |||||||
| VAS14_22994 glutamine synthetase | 0.6 | 0.08 | 130 | 19 | 0.04 | 0.01 | GINERDMVMMPDASSAVLDPFTEDATLNIR |
| 0.41 | 0.68 | IHPGEAMDKDLYDLPAEEAAKIPTVAESLQGALQALSDDR | |||||
| 0.5 | 0.004 | FGDPAANPYLAFAAMLMAGLDGIQNK | |||||
| 0.58 | 0.58 | IHPGEAMDKDLYDLPAEEAAEIPTVVESLQGALQALSDDR | |||||
| 2.47 | 0.3 | IHPGEAMDKDLYDLPAEEAAEIPTLAESLQGALQALSDDR | |||||
| 2.65 | 0.25 | IHPGEAMQKDLYDLPAEEAAEIPTVAESLQGALQALSDDR | |||||
| 2.69 | 0.3 | AEIPTVAESLQGALQALSDDR | |||||
| VAS14_19296 putative ribosomal | 1.45 | 0.02 | 41 | 5 | 0.14 | 0.01 | HTGSQVYVQPASEGTGIIAGGAM[DTM]R |
| subunit protein S5 | |||||||
| VAS14_19191 elongation factor Tu | 1.32 | 0.05 | 200 | 25 | 0.04 | 0.03 | ELLSEYDFPGDDAPVIMGSALGALNGEK |
| 6.12 | 0.01 | ELLSEYDFPGDDAPVIMGSALGALNGEK |
Protein ratio refers to the differential abundance of the protein between UVB and dark treatments. Peptide ratio refers to the differential abundance of the carbonylated peptides. Peptides were scored as either carbonylated or not irrespective of whether multiple residues in the peptide were carbonylated. Data is for the combination of all replicates.
*
Stars show proteins with similar trends for peptide and protein ratios (UVB/dark ratio <1 or >1). Bold residues in the sequence of peptides indicate sites of carbonylation.