Figure 4. TrkB receptor is involved in BDNF-mediated migration of human chondrosarcoma cells.

(A&B) Cells were pretreated with K252a (50 nM) or TrkB monoclonal antibodies (5 µg/ml) for 30 min or transfected with TrkB shRNA for 24 h and then allowed to migrate for 24 h toward BDNF (50 ng/ml)-containing medium. The in vitro migration and wound healing activity was measured (n = 5). (C&D) Cells were pretreated with K252a (50 nM) or TrkB mAb (5 µg/ml) for 30 min followed by stimulation with BDNF, and mRNA and cell-surface β5 integrin expression was examined using qPCR and flow cytometry (n = 4). Results are expressed as the mean ± SEM. *, p<0.05 compared with the control. ^#, p<0.05 compared with the BDNF-treated group.