Table 2. Primer pairs used for quantitative real-time PCR.

Gene name (Abbreviation)	Accession No.	Primer Namea	Sequence (5′-3′)	Product length (bp)	Tm (°C)	Primer efficiency (%)	R2 b
Elongation factor-1	DQ192234	SlN-F1	CTCCTACATCAAGAAGATC	295	55	96.7	0.997
(EF1)		SlN-R1	CTTGAGGATACCAGTTTC		55
Ribosomal protein L10	KC866373	SlN-F2	GACTTGGGTAAGAAGAAG	189	55	109.7	0.998
(RPL10)		SlN-R2	GATGACATGGAATGGATG		55
Actin	DQ494753	SlN-F3	GATCATGTTTGAGACCTT	214	55	107.3	0.998
(ACTB)		SlN-R3	GATCTTCATGAGGTAGTC		55
Glyceraldehyde-3-phosphate dehydrogenase	HQ012003	SlN-F4	GGGTATTCTTGACTACAC	184	55	109.6	0.996
(GAPDH)		SlN-R4	CTGGATGTACTTGATGAG		55
Beta FTZ-F1	HQ260326	SlN-F5	CTGATGAGACTACACTTC	297	55	107.9	0.998
(FTZF1)		SlN-R5	CAGGAACTACCATTACTAG		55
Ubiquinol-cytochrome c reductase	HQ599193	SlN-F6	GCCAAGATTGAGATCAAG	204	55	109.7	0.998
(UCCR)		SlN-R6	GCATACTCCGATAACTAC		55
Ribosomal protein S3	KC866374	SlN-F7	CGGAGATCATCATTATGG	191	55	105.6	0.997
(RPS3)		SlN-R7	GAGTTTGTATCTGAGAGAC		55
Arginine kinase	HQ840714	SlAK-F	CTGAAGAAGTACCTTACC	80	55	105.2	0.989
(AK)		SlAK-R	CAATCCAGCAGAGTTGAG		55

^aF and R refer to forward and reverse primers, respectively;

^b R ² refers to the coefficient of determination.