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. 2013 Jul 9;8(7):e68026. doi: 10.1371/journal.pone.0068026

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© 2013 Resende et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Example of an apoptotic hub cell found after RNAi-mediated reduction in hdc. DN-cadherin (red), Apotag (green), Genotype: updGal4;UAS-hdcRNAi¹;Gal80^ts. (B) Example of a 1d updGal4;UAS-hdcRNAi¹ testis containing a residual hub composed of a single hub cell (*), Hub stained with both FasIII (red) and DE-cadherin (green), DAPI (DNA, blue). (C) Co-expression of p35 rescues the strong phenotype observed with hdcRNAi¹. Compare to (B); (D) Hub cell number quantifications of different genotypes/time points; Mean and SD are shown; Welch’s t-test (***P<0.001). (E) Example of a compromised 10d updGal4;UAS-DIAP2RNAi testis; Note that DE-Cad shows two hub cells but one of them already lost FasIII staining); (F) Apotag positive hub cell in a updGal4;UAS-DIAP2RNAi testis; Scale bars 20 µm.