Table 1. Results of candidate gene screen for regulators of hub size.
| Genotype | hub cell number | +/− std dev |
| 1d | 10d | |
| updGal4;+ (control) | 10.06+/−2.31 (N = 31); 0% | 10.94+/−2.45 (N = 31); 0% |
| updGal4;UAS-LacZ (control) | 10.18+/−2.46 (N = 33); 0% | 10.93+/−2.07 (N = 30); 0% |
| UAS-hdcRNAi1/+ (control) | 8.53+/−1.48 (N = 30); 0% | 10.19+/−2.83 (N = 16); 0% |
| updGal4;UAS-Zfh1RNAi | 11.79+/−1.88 (N = 28); 0% | 11.07+/−2.40 (N = 29); 0% |
| updGal4;UAS-hdcRNAi1 | 0.43+/−0.73 (N = 30); 67% | ND |
| updGal4;UAS-hdcRNAi2 | 10.22+/−1.73 (N = 27); 0% | 4.20+/−1.44 (N = 25); 0% |
| updGal4;UAS-hdcRNAi3 | 10.30+/−2.37 (N = 30); 0% | 5.17+/−2.27 (N = 35); 0% |
| updGal4;UAS-hdcRNAi1;Gal80ts * | 7.04+/−2.12 (N = 27); 0% | 1.6+/−1.36 (N = 26); 35% |
| updGal4;UAS-hdcRNAi2 ? | 12.78+/−2.73 (N = 18); 0% | 8.34+/−2.74 (N = 26); 0% |
| updGal4;UAS-hdcRNAi3 ? | 11.81+/−2.76 (N = 26); 0% | 9.04+/−1.80 (N = 23); 0% |
| updGal4;UAS-hdcRA/C;Gal80ts # * | ND | 9.28+/−3.21 (N = 29); 0% |
| updGal4;UAS-hdcGsd404;Gal80ts # * | ND | 8.79+/−2.15 (N = 28); 0% |
| updGal4;;UAS-UnkRNAi | 9.69+/−1.99 (N = 16); 0% | 8.71+/−2.91 (N = 17); 0% |
| updGal4;UAS-CycKRNAi | 11.08+/−1.73 (N = 12); 0% | 6.24+/−2.94 (N = 33); 0% |
| updGal;UAS-reaper | Embryonic lethal | Embryonic lethal |
| updGal4;UAS-reaper;Gal80ts * | 8.96+/−2.40 (N = 23); 0% | 9.07+/−2.08 (N = 29); 0% |
| updGal4;;UAS-hid,UAS-reaper | Embryonic lethal | Embryonic lethal |
| updGal4;;UAS-hid,UAS-reaper/Gal80ts * | 9.08+/−1.56 (N = 23); 0% | 5.15+/−1.49 (N = 26); 0% |
| updGal4;UAS-DIAP2RNAi | 3.80+/−2.20 (N = 36); 6% | 1.40+/−1.82(N = 20); 45% |
Average number of hub cells (± S.D.) in testes from 1 or 10-day old males of designated genotypes. Percentage of testes with total hub cell loss also shown. See Methods for screen conditions.
*
Flies raised at 18°C and shifted to 29°C upon eclosion.
?Flies raised at 18°C and shifted to 25°C upon eclosion.
#
Lines used for hdc over-expression.