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In vitro validation of predicted FOXA2 bivalency. Immunoprecipitation of H3K4me3, H3K27me3 and total H3 from chromatin of pluripotent SA461s and H9s revealed enrichment of the histone modifications at the FOXA2 transcriptional start site, as identified by quantitative PCR using primers for three regions, covering around 2.5 kb in total. *P <0.05 versus IgG control.
