Table 1.

Summary of the Cell Expansion Experiment in Conventional Cell Culture Flasks and in the Bioreactor System

	Static expansion (flask)	Dynamic expansion (bioreactor)
Growth surface area [cm2]	25	6000
Seeded cell density [1/cm2]	1500	1500
Seeded cell number [-]	37500	9000000
End cell density [1/cm2]	47000 ± 4435	58013 ± 9208
Harvested cell number [-]	1.19 ± 0.11 × 106	348.08 ± 55.25 × 106
Cultivation time [h]	216	216
Expansion [fold]	31.8 ± 3.0	38.7 ± 6.1
Doubling time[h]	43.3 ± 1.6	41.0 ± 1.7
Maximal growth rate µmax [h-1]	0.022 ± 0.0036	0.023 ± 0.0023
Maximal growth rate µmax [d-1]	0.53 ± 0.087	0.560 ± 0.056
Lactate production (mmol/d)/glucose consumption (mmol/d) [-]	1.63 ± 0.33	1.82 ± 0.05