Figure 2.
Angiogenic signaling from mono- and cocultured constructs and the vascular host response. (A) Rt2 Profiler Angiogenesis PCR Array after 1 week of dynamic culture in vitro. Green squares represent genes expressed higher in monocultured MSCs, and red squares represent genes expressed higher in MSC/EC constructs. Fold changes greater than three are indicated with green or red font, respectively. (B) Corresponding VEGF of mouse origin was higher in monocultured constructs at 1 week of implantation. However, the difference was not statistically significant. (C) No difference in expression of VEGF from implanted cells was observed at 1 week, whereas an upregulation was found at 3 weeks. *P < 0.05; n = 6. (D) Expression of mouse CD31 was slightly higher at both time points for monocultured constructs, but the differences were not statistically significant.