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. 2013 May 9;27(7):1078–1090. doi: 10.1210/me.2012-1346

Figure 2.

Figure 2.

Under nonoxidative conditions p38 MAPK mediates MafA degradation through the proteasomal pathway. A, MIN6 cells cotransfected with pcDNA or WT-MafA:HSV along with Ub:HA expression plasmids were cultured in 0.8 mM glucose for the last 16 hours in the presence of indicated combination of DMSO, 20 μM SB203580 (SB20), 20 μM SB216763 (SB21), and 10 μM MG132. Whole-cell extracts were immunoprecipitated (IP) with α-HSV antibody followed by immunoblotting (IB) with α-HA antibody. A representative gel from at least 3 independent experiments is shown. B, MIN6 cells cotransfected with pcDNA, WT-MafA:HSV, A57A134-, A65-, or A57A65A134-HSV-tagged MafA and Ub:HA expression plasmids were cultured in 0.8 mM glucose for the last 16 hours in the presence of 10 μM MG132. Whole-cell extracts were immunoprecipitated with α-HSV antibody followed by immunoblotting with α-HA antibody. The reblot with α-HSV shows correct mobility and levels of different MafA derivatives. A representative gel from at least 3 independent experiments is shown.