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. 2013 Jun 6;27(7):1048–1064. doi: 10.1210/me.2012-1322

Figure 3.

Figure 3.

Figure 3.

Collagens and PRL-Dependent Tyrosyl Phosphorylation of PAK1 Regulate Secretion of Invasive-Relevant MMPs. TMX2–28 cells stably overexpressing GFP, PAK1 WT, or PAK1 Y3F were plated on plastic, on plastic covered with collagen I, embedded in collagen I, on plastic covered with collagen IV, or embedded in collagen IV. After 24 hours of serum deprivation, the cells were treated with (black bars) or without (white bars) 100 ng/mL PRL for 48 hours, and the conditioned medium was assessed for protein level of secreted MMP-1 (A), MMP-3 (B), MMP-2 (C), and MMP-9 (D) by ELISA. E, TMX2–28 cells were transfected with control or PAK1 siRNA, stimulated (black bars) or not (white bars) with PRL, and assessed for secretion of MMPs as in panels A–D. Bars represent mean ± SE. *, P < .05 compared with the same cells untreated with PRL. #, P < .05 compared with cells expressing GFP with the same treatment. Each experiment was repeated 3 times.