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The expression of Nestin during the induction process among the three groups. The quantity of mRNA of Nestin in RA plus serum-free medium group was the highest among the three groups (A). During the induction process, Nestin-positive cell rate was the highest in RA plus serum-free-medium group (B) (*compared with the spontaneous-differentiation group, P < 0.05; #compared with the RA-induction group, P < 0.05).
