Figure 1.

Leptin and leptin receptor (Ob-R) expression in various breast cancer cell lines. (A) Notably, there is increased leptin expression in estrogen receptor (ER)-positive cell lines (MCF-7, T-47D, and HCC-70) compared to other types of breast cancer cells. β-Actin was used for loading control in both cell lines. (B) In breast cancer cell lines, the Ob-R long (Ob-Rb) is the predominat receptor expressed compared to normal breast tissue, whereas there is no difference in Ob-R short (Ob-Ra) expression between breast cancer cell lines and normal breast tissue. Top, expression by Western blot assay; Bottom, quantitative analysis of Western blot results using densitometry.