Table 1.
Some stem cell-related genes are transcribed similarly in MDSCs, irrespective of myostatin inactivation
| Gene | Function | WT | KO |
|---|---|---|---|
| Myc | Myelocytomatosis oncogene | 12.4 | 18.1 |
| Pou5F1 | Pou domain (Oct4) | 10.1 | 16.7 |
| Akp 2 | Alkaline phosphatase 2 | 6.4 | 6.9 |
| Akp 5 | Alkaline phosphatase 5 | 1.2 | 1.6 |
| Tert | Telomerase reverse transcriptase | 1.0 | 1.0 |
| Utf 1 | Undifferentiated embryonic cell TP1 | 1.0 | 0.8 |
| Man 1 | Mastermind-like 1 | 13.1 | 16.7 |
| Lif | Leukemia inhibitory factor | 1.5 | 0.9 |
| PPARγ | Peroxisome proliferator-activated receptor γ | 1.1 | 1.8 |
RNA from the two MDSC types at 80% confluence (not undergoing myogenesis) were treated by DNAse and submitted to DNA microarrays. Some key stem cell genes are selected. Values are relative expression levels normalized by housekeeping genes. GAPDH expression was 98 to 102. MDSC, muscle-derived stem cell; WT, wild type; KO, myostatin knockout.