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The tube formation assay for induced CD31-CD146-SP cells 21 days after induction at passage 3 to 4 and non SP cells. An extensive network of cords and tube-like structures can be seen on Matrigel by induced CD31-CD146-SP cells after 48 hours (A, C), and only short strands formed in non-SP cells after 48 hours (B, D). The experiment was repeated three times, and one representative experiment is presented. SP, side population.
