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. 2013 Jul 10;4:236. doi: 10.3389/fpls.2013.00236

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Copyright © 2013 Bustos-Sanmamed, Bazin, Hartmann, Crespi and Lelandais-Brière.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Phylogenetic tree of AGO proteins in M. truncatula, L. japonicus, G. max, A. thaliana, P. trichocarpa, and O. sativa. Full length sequences of the predicted AGO proteins from A. thaliana, P. trichocarpa and O. sativa were retrieved from Genbank (NCBI). DCL from M. truncatula, soybean (G. max) and L. japonicus were searched by tBLASTX in genomic sequence databases: legumes Lotus japonicus (Miyakogusa.jp 2.5, Sato et al., 2008), Medicago truncatula (Mt 3.5.1, Young et al., 2011), and Glycine max (Glyma1.181, http://www.plantgdb.org/GmGDB/), and named according to their similarity with A. thaliana proteins or according to previous publications (correspondances given in Data Sheet 3). Phylogenetic trees were constructed thanks to T-Coffee software. The phylogenetic tree was generated with MEGA4.0 software using the Neighbor joining tree (Capitão et al., 2011). The four AGO clades are indicated in the right part of the figure.