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. 2013 Jun 28;5(2):75–84. doi: 10.1038/ijos.2013.41

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Copyright © 2013 West China School of Stomatology

This work is licensed under the Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Lineage studies by mapping Cre activity in the root region of a second molar in a 2-week-old mouse by confocal microscopy using the Olympus FV 1000. Combined DAPI-stained nuclei (blue), Sp7-Cre-EGFP⁺ (green) and tdTomato⁺ (red) cells are shown. (a) DIC image. (b) Sp7⁺ EGFP signal. (c) tdTomato signal representing Cre event and cells derived from the Cre events. AP, apical papilla; BV, blood vessel; DAPI, 4′,6-diamidino-2-phenylindole; DIC, differential interference contrast; Dp, dental-pulp chamber; Od, odontoblasts; PDL, periodontal ligament region.