Figure 5. SAXS analysis for t-PTEN, n-PTEN and 4p-PTEN.
(A) Scattering diagrams and (B) pair distribution function (Pofr) plots for t-PTEN, n-PTEN, and 4p-PTEN. (C) The molecular envelopes for t-PTEN, n-PTEN, and 4p-PTEN overlaid with the tailless crystal structure containing the phosphatase domain (blue), C2 domain (magenta), and CBRIII loop (yellow). (D) Summary of Rg and Dmax values for t-PTEN, n-PTEN, and 4p-PTEN.
Figure 5—figure supplement 1. Molecular envelopes of t-PTEN, n-PTEN and 4p-PTEN obtained from SAXS analysis.
Molecular envelopes of t-PTEN, n-PTEN, and 4p-PTEN were generated from SAXS scattering data using the ab initio modeling program DAMMIN. Outputs from ten DAMMIN runs were averaged for each protein using DAMAVER and are shown overlaid with the tailless crystal structure with phosphatase domain shown in blue, the C2 domain shown in magenta and the CBRIII loop shown in yellow. Front, side and top views are shown for each protein.