Figure 7. In trans peptide inhibition and binding of PTEN.

(A) Tail peptide inhibition of t-PTEN with either n-25mer or 4p-25mer. (B) K_m curve of t-PTEN in the absence (k_cat = 2.9 ± 0.1 min⁻¹; K_m = 33 ± 2.1 μM, k_cat/K_m = 0.088 ± 0.004 min⁻¹μM⁻¹) or presence (k_cat/K_m = 0.006 ± 0.0003 min⁻¹μM⁻¹) of 1 μM 4p-25mer phosphopeptide. (C) Reduced inhibition of D5 t-PTEN mutant in the presence of 4p-25mer peptide. (D) Vesicle sedimentation of t-PTEN in absence and presence of 10 μM tail peptides. Data points are shown as the mean ± the SEM of three experiments performed in duplicate.

DOI: http://dx.doi.org/10.7554/eLife.00691.024