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. Author manuscript; available in PMC: 2013 Jul 10.
Published in final edited form as: Nat Methods. 2012 Sep 30;9(11):1095–1100. doi: 10.1038/nmeth.2182

Figure 4. Specificity constants of individual substrates can be calculated from the MSP-MS assay.

Figure 4

(a) The precursor ion abundance of KARSAFAEnWPDHN (black) and cleaved product (grey) at four time intervals after addition of Granzyme B to the MSP-MS assay. The precursor ion abundance was calculated using Xcalibur. (b) Progress curves of product formation for peptides KHPLETVYAD and KARSAFAEnWPD. In each case, the parent substrates were completely hydrolyzed by Granzyme B within 1200 minutes, therefore the concentration of each product is known and kinetic values were subsequently calculated.