Figure 2.

Plitidepsin increases the level of phosphorylated JNK in tumors of K562-bearing mice. Mice xenografted with K562 cells received a single administration of plitidepsin (APL; 200 μg/kg) and either immediately (0 min) or at the indicated times after injection were sacrificed (5 mice/group). (A) Western blot analysis of total (JNK) and phosphorylated JNK (p-JNK) in excised tumors. Actin was used as loading control. K, K562 cells; K + APL, plitidepsin-treated K562 cells. (B) Quantification of p-JNK levels (normalized to those of total JNK) and related to that of vehicle-treated K562 sample at the indicated times after APL administration. Mean ± SEM values of each group of animals are shown.