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. 2013 May 21;11(5):1677–1692. doi: 10.3390/md11051677

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Plitidepsin increases JNK phosphorylation in rat peripheral mononuclear blood cells. (A) Blood mononuclear cells (PBMCs) were isolated from non-tumor-bearing rats after 4, 8 and 24 h i.v. administration of plitidepsin (1 mg/kg) and the levels of total (JNK) and phosphorylated JNK (p-JNK) were analyzed by Western blot. Control group were treated only with vehicle (control (0 h)). Actin was used as loading control for normalization. Immunoblots were incubated with infrared-labeled secondary antibodies, and the signals were visualized and quantified using the Odyssey Infrared Imaging System (Li-Cor Biosciences). (B) Quantification of the p-JNK/JNK ratio upon normalization to actin levels at the indicated times after plitidepsin administration in PBMCs derived from non-tumor-bearing rats. Mean ± SEM values of each group of animals are shown.