Skip to main content
. 2013 May 21;11(5):1677–1692. doi: 10.3390/md11051677

Figure 6.

Figure 6

Plitidepsin induces a statistically significant increase of the level of phosphorylated JNK in the PBMCs of healthy rats after 8 h of administration. (A) Western blot analysis of JNK and p-JNK proteins in PBMCs obtained from rats treated with plitidepsin (1 mg/kg) for 8 h. Actin was used as loading control. Immunoblots were incubated with infrared-labeled secondary antibodies, and the signals were visualized and quantified using the Odyssey Infrared Imaging System (Li-Cor Biosciences). (B) Statistical analysis of JNK activation in PBMCs obtained from rats at 8 h after plitidepsin administration. Comparison between the p-JNK/JNK ratio in PBMCs of rats treated with plitidepsin or vehicle for 8 h using the Wilcoxon rank-sum test. A box-plot of JNK activation in each group (15–18 mice/group) and its statistical significance using Wilcoxon rank-sum test (p) are presented. Boxes in the plot include values in the 25%–75% interval; internal lines represent the median.