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. 2013 Jun 24;2013:459271. doi: 10.1155/2013/459271

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Copyright © 2013 Chih-Wen Chi et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of Armillaridin on mitochondrial transmembrane potential reduction, apoptosis, and caspase inhibitor activity in CE81T/VGH cells. (a) Representative mitochondrial transmembrane potential histograms were shown as CE81T/VGH cells treated with Armillaridin for 24 hours. (b) Relative level of mitochondrial transmembrane potential. (c) The amount of cells with early apoptosis (FITC⁺/PI⁻ cells) by the Annexin V/PI staining assay. (d) Caspase 3 activity with or without pretreatment with pan caspase inhibitor at day 1 (closed column) and day 2 (opened column). Results are mean ± SE from three independent experiments. Significant differences between control cells and cells treated with AM 20 μM are indicated by *P < 0.001. AM: Armillaridin.