Figure 1.

LZ-8 stimulates Treg expansion in murine and human CD4⁺ T cells. Primary murine and human CD4⁺ T cells were cultured for 72 h, either in the absence of stimulation as the control (CTR), in the presence of stimulation with recombinant LZ-8 antibody (rLZ-8; 1 μg/mL), or in the presence of stimulation with anti-CD3/28 antibodies (CD3/CD28; 1 μg/mL each, (a, b). The cells were then harvested for flow cytometry analysis of CD25, FOXP3, and CTLA-4 expression. (c and d) The levels of IL-2, IL-10, IFN-γ, and TGF-β in the culture supernatants were measured by ELISA. (e) The cell proliferation of CD4⁺ T cells treated with or without PHA (2 μg/mL) and then cocultured with indicated CD4⁺ T cells was monitored by cell-trace CFSE fluorescence via flow cytometry.