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. 2013 Jun 25;2013:981012. doi: 10.1155/2013/981012

Table 1.

Technical data for the analysis of single nucleotide polymorphisms (SNPs) in the human IL-13 gene.

Region Primers PCR product size (bp) Restriction enzyme* Annealing temperature Reference
Promoter:
(−1111C>T)
Forward:
Reverse:
5′-ACT TCT GGG AGT CAG AGC CA-3′  
5′-TAC AGC CAT GTC GCC TTT TCC TGC TCT TCC GTC-3′
372 Hpy99I 60°C [22]

Coding:
(4257G>A)
Forward:
Reverse:
5′-CTT CCG TGA GGA CTG AAT GAG ACG GTC-3′  
5′-GCA AAT AAT GAT GCT TTC GAA GTT TCA GTG GA-3′
236 NlaIV 60°C [23]

GenBank accession number U31120 was used as the reference sequence.

*New England Biolabs, Beverly, MA, USA.