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. Author manuscript; available in PMC: 2013 Oct 10.
Published in final edited form as: J Am Chem Soc. 2012 Sep 25;134(40):16717–16724. doi: 10.1021/ja3067429

Figure 2.

Figure 2

Cleavage of the 5′-PO or 5′-PS substrate by some hairpin ribozyme variants.

The substrate strand was [5′-32P]-labelled, and reaction products were separated by electrophoresis in 20 % polyacrylamide and subjected to phosphorimaging.

A. Gel electrophoresis. The 5′-PO (tracks 1, 3, 5, 7 and 9) and 5′-PS (track 2, 4, 6, 8 and 10) substrates were incubated with no ribozyme (track 1, 2), the natural hairpin ribozyme (track 3, 4), A38P (track 5, 6), A10U (7, 8) or ΔB ribozyme where loop B was removed by complementation (track 9, 10) for 20 min. under standard conditions.

B. Reaction progress of the cleavage reaction under standard conditions, fitted to double exponential functions. Open circles: 5′-PS + A38P ribozyme, filled squares: 5′-PO + natural ribozyme, filled triangles and filled circles (inset): 5′-PO + A38P ribozyme.