TABLE 2.
Organelle | wt MV | Loa/+ MV | Loa/+ RtTg | |||
---|---|---|---|---|---|---|
Mean | ±SEM | Mean | ±SEM | Mean | ±SEM | |
Total Cytosol | 55.9 | 59.1 | 43.0 | |||
Cytosol without polyribosomes | 25.1 | ±6.3 | 59.1** | ±3.3 | 25.3 | ±4.4 |
Cytosol with polyribosomes | 30.8 | ±0.3 | 0.0* | ±0.0 | 17.7 | ±0.4 |
Endoplasmic reticulum | 8.9 | ±3.2 | 2.15* | ±0.2 | 11.9 | ±0.4 |
Total Mitochondria | 10.3 | ±0.4 | 12.5 | ±1.5 | 8.5 | ±0.2 |
Normal Mitochondria | 9.2 | ±1.5 | 3.75* | ±0.3 | 7.5 | ±0.1 |
“Aged” Mitochondria | 1.1 | ±1.1 | 8.75** | ±1.2 | 1.0 | ±0.1 |
Early endosomes | 2.8 | ±0.4 | 4.65 | ±1.2 | 4.6 | ±1.8 |
Late endosomes | 3.4 | ±1.0 | 1.8* | ±0.5 | 3.2 | ±0.2 |
Multivesicular bodies | 2.0 | ±1.2 | 0.25* | ±0.2 | 1.6 | ±0.5 |
Lysosomes | 1.6 | ±1.6 | 0.0* | ±0.0 | 2.6 | ±1.7 |
Tubules | 1.7 | ±0.6 | 1.9 | ±0.5 | 1.8 | ±0.7 |
Plasma membrane | 7.6 | ±1.2 | 8.8 | ±0.1 | 8.8 | ±3.3 |
Synapses | 5.8 | ±0.5 | 8.3 | ±2.2 | 13.1 | ±1.2 |
Unidentified | 0.0 | ±0.0 | 0.55 | ±0.1 | 0.9 | ±0.8 |
100.0 | 100.0 | 100.0 |
MV, Trigeminal Motor neurons; RtTg, reticulotegmental nucleus.
Major differences in bold:
decrease;
increase.