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The fitness of cells harboring the Ti plasmid increased as the level of available octopine increased (t = 18.35, p < 0.0001). Utilizable nitrogen levels limited growth of the population and was available in the form of (NH4)2SO4 and to varying levels octopine. Both pTi− and pTi+ cells catabolized the (NH4)2SO4, however only pTi+ cells could catabolize octopine. Values are LS mean ± standard error of six replicates.
