TABLE 4.

STAGE III REPLICATION RESULTS FOR THE TWO IL1RN SINGLE-NUCLEOTIDE POLYMORPHISMS THAT DEMONSTRATED AT LEAST MARGINAL ACUTE RESPIRATORY DISTRESS SYNDROME ASSOCIATION IN STAGES I AND II

SNP	HWE P Value	ARDS MAF	No ARDS MAF	Stage III OR (95% CI)	Stage III P Value
rs315952C	0.018	0.24	0.28	0.83 (0.71–0.96)	0.015
rs380092T	0.073	0.28	0.31	0.83 (0.72–0.97)	0.017

Definition of abbreviations: ARDS = acute respiratory distress syndrome; CI = confidence interval; HWE = Hardy-Weinberg equilibrium; MAF = minor allele frequency; OR = odds ratio; SNP = single-nucleotide polymorphism.

Results for loci which failed to replicate their association in stage II are shown in the online supplement (see Table E5). The α level to declare significance in stage III was less than 0.05. Both stage I IL1RN variants, rs315952 and rs380092, replicated their associations with decreased risk of ARDS in the mixed intensive care unit population, for whom sepsis was the most common risk factor for ARDS. HWE P values are the result of the chi-square test for HWE proportions in the overall population.