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. 2013 May;48(5):655–664. doi: 10.1165/rcmb.2012-0423OC

Figure 7.

Figure 7.

IL-1β at sensitization is sufficient to elicit IL-17A production by CD4+TCRβ+ T cells at antigen challenge. For antigen sensitization, wild-type (WT) C57BL/6 mice received IL-1β or 0.1% BSA in saline (control) by intranasal instillation before OVA nebulization on Day 1. Mice were again exposed to OVA on Days 2 and 3. Mice were OVA-challenged on Days 14, 15, and 16, and analyzed on Day 17. (A) Total BAL neutrophils were enumerated. (B) Lung single-cell suspensions were restimulated in the presence of OVA antigen, and IL-17A was measured by ELISA. Lung single-cell suspensions were stimulated in PMA and ionomycin for 3 hours before staining for intracellular IL-17A. (C) The percentage of IL-17A+ cells within the CD4+TCRβ+ gate is shown. *P < 0.05, according to unpaired Student t test (n = 5/group).