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. 2013 May 2;134(2):291–303. doi: 10.1093/toxsci/kft104

Fig. 2.

Fig. 2.

BDCM exposure in obese mice causes hepatic inflammation, Kupffer cell activation, TGF-β expression, and increased leptin release. (A) qRTPCR analysis of liver IL1-β, IFN-γ, TNF-α, CD68 (Kupffer cell activation marker), TGF-β, Pan macrophage marker F4/80, and leptin mRNA expression of DIO, DIO + BDCM, and CYP2E1 KO + BDCM mice. Y-axis represents fold of mRNA expression when compared against DIO-only group, n = 4. (B) Immunohistochemistry of liver slices of DIO, DIO + BDCM, and CYP2E1 KO + BDCM for CD68 (panels i–iii) and TGF-β (panels iv–vi) showing ×20 images. (C) Western blot analysis of adipokine leptin from liver homogenates of DIO, DIO + BDCM, and CYP2E1 KO + BDCM groups. (D) Immunoreactive band analysis of leptin normalized against beta actin. Y-axis depicts the leptin/actin ratio from DIO, DIO + BDCM, and CYP2E1 KO + BDCM groups (n = 3). *p < 0.05 is considered statistically significant.