Fig. 3.

As(III) increases lipolysis in adipocytes. Adipocytes differentiated from hMSC were incubated with or without 1μM As(III) for 72h, and medium above the cells was sampled at the indicated times for glycerol content as a measure of lipolysis (control was sampled at 72h). Released glycerol was measured using a colorimetric assay, as described in Materials and Methods section, and data are presented as mean ± SEM glycerol released in three separate cultures and from two independent experiments (each sample assayed in duplicate). Data were analyzed for significance by ANOVA and Newman-Keuls post hoc test for differences between groups (**p < 0.01 from control).