Fig. 6.

Effect of Ptx on As(III)-exposed adipocytes. Ptx (1μM) was added to the indicated groups of differentiated adipocytes for 24h before 1μM As(III) was added. After 72h, medium was collected for glycerol measurements, and the cells were harvested for protein and RNA isolation. (A) Total proteins from cell lysates were probed by Western analysis for PLIN1 and β-actin levels, and the graph presents densitometric analysis from six cultures in each group (mean ± SEM of fold difference from untreated cells [control]). (B) Total RNA was assayed by qRT-PCR for PLIN1, ADIPOQ, or RPL13A transcript levels. Data are mean ± SEM of at least two independent experiments. (C) Released glycerol was quantified and data are presented as mean ± SEM μg of glycerol released per ml of medium in three separate cultures and from two independent experiments (each sample assayed in duplicate). Data were analyzed by one-way ANOVA and Bonferroni’s post hoc test for significance (*p < 0.05, **p < 0.01).