Fig. 6.

Western blot analysis of the effects of treatment with saline (control), deprenyl, and/or hydroxyurea (HU, 400 or 600mg/kg) on cleaved caspase-3 in GD 9 embryos. (A) Western blots depict cleaved caspase-3(~17kDa), with β-actin as a loading control. (B) Quantification of Western blots. The data represent means ± SEM (n = 4 separate experiments, with 8–15 embryos per sample). Two-way ANOVA and a post- hoc Bonferroni correction were done. Asterisks (*) and (**) denote a statistically significant difference (p < 0.05) and (p < 0.01), respectively. † denotes a significant difference between the hydroxyurea-treated groups in the presence or absence of deprenyl (p < 0.05).