Table 1. PacBio correction results.

Corrected (PBcR) read accuracy as compared to reference sequence. Reads were mapped using Nucmer 3.23.⁵⁰ For all statistics, only reads > 500 bp were included. %TP (Throughput): the percentage of raw uncorrected bases that are in non-chimeric, correctly trimmed sequences after correction. %Idy (Identity): average identity of good corrected reads to the reference. %Cov (Coverage): average coverage of good corrected reads by a single match to the reference. %Chimer: the percentage of corrected bases within reads with a split mapping to the reference. %Trim: the percentage of corrected bases within reads with a single match to the reference over less than 99.5% of their length. The corrected sequences remain above 99% identity and 99% trim within the repetitive regions of the genome (Supplementary Table S3).

Organism	% TP	% Idy (Reads)	% Idy (Assembly)	% Cov	% Chimer	% Trim	Time (s)	Mem (GB)
Lambda NEB3011	74.03%	99.90%	100.00%	100.00%	1.82%	0.10%	121	0.12
E. coli K12	57.46%	99.99%	99.99 %	99.92%	2.02%	0.34%	1580	2.10
S. cerevisiae S228c	21.86%	99.90%	99.97%	99.93%	1.46%	0.33%	4357	5.90