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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Chem Biol Drug Des. 2013 Jul;82(1):106–118. doi: 10.1111/cbdd.12138

Figure 1.

Figure 1

Competitive binding of peptide 6 with FITC-labeled antibody to CD58 protein (anti-CD58 is known to bind to adhesion domain of CD58) on Caco-2 cells expressing CD58 protein. Inhibition of antibody binding to Caco-2 cells at different concentrations of peptide 6 is shown. Relative fluorescence intensity was represented. Approximately 1 × 104 Caco-2 cells/well were coated on 96-well plates. After cells achieved confluence, they were incubated with FITC-AbCD58 (+ signs, pre-diluted, 20 µL) and/or peptide 6 for 1 h. After washing three times, medium was added and fluorescence was read using a microplate reader at excitation λ 485 nm and emission λ 528 nm. Fluorescence from the blank was subtracted for representation. Data are from triplicate experiments.