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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Chem Biol Drug Des. 2013 Jul;82(1):106–118. doi: 10.1111/cbdd.12138

Figure 2.

Figure 2

Competitive binding of peptide 6 with FITC-labeled antibody to Caco-2 cells expressing CD58 monitored by flow cytometry (BD FACS Calibur). 10,000 cells were counted using green fluorescence. The figure represents forward and side scatter representation of Caco-2 cells. A) Caco-2 cells without peptide or antibody. More than 85% of the unstained cells are observed in the lower left quadrant. B) Cells with FITC-AbCD58. There was a forward shift and more than 82% of stained cells were observed in the lower right quadrant. C, D, E, and F) Cells with peptide 6 and FITC-AbCD58 at peptide concentrations of 1, 50, 100, and 200 µM, respectively. Notice that in the presence of peptide 6, more Caco-2 cells without antibody labeling (cell population shifted to left compared to B) were observed, suggesting the inhibition of FITC-AbCD58 to Caco-2 cells.