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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Chem Biol Drug Des. 2013 Jul;82(1):106–118. doi: 10.1111/cbdd.12138

Figure 3.

Figure 3

Competitive binding of fluorescently labeled peptide 6 (5FAM-peptide 6) with anti-CD58 to Caco-2 cells expressing CD58 monitored by flow-cytometry. 10,000 cells were counted using green fluorescence. The figure represents forward and side scatter representation of Caco-2 cells. A) Caco-2 cells without peptide or antibody. B) Cells with 5FAM peptide 6, 100 µM. C) Cells with 5FAM-peptide 6, 100 µM, and anti-CD58. More than 75% of the cells were stained with peptide 6. D) Cells with 5FAM peptide 6, 200 µM, and anti-CD58. More than 80% of the cells were stained with peptide 6.