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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Chem Biol Drug Des. 2013 Jul;82(1):106–118. doi: 10.1111/cbdd.12138

Figure 8.

Figure 8

Immunogenicity of peptide 6 in HLA transgenic mice expressing human HLA-DQA1*0301, DQB1*0302 (DQ8) gene but lacking all four chains of mouse endogenous class II molecules. LNCs (1 × 106) from transgenic mice were cultured. Con A (20 µg/mL, positive control) and varying concentrations of peptide 6 and control peptide. The cells were incubated for 48 h at 37 °C. During the last 18 h, the cells were pulsed with 3H-thymidine (1 µCi/well). At the end of the assay, the cells were harvested using a plate harvester, and incorporated radioactivity was determined using an automated counter (Microbeta, Perkin Elmer Wallac). The results are depicted as delta cpm. There was no significant difference in response when challenged in vitro by the control peptide or peptide 6, suggesting that peptide 6 is not immunogenic.