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. Author manuscript; available in PMC: 2013 Jul 10.
Published in final edited form as: Methods Mol Biol. 2010;594:409–423. doi: 10.1007/978-1-60761-411-1_29

Fig. 29.3.

Fig. 29.3

Oxidative stress promotes the binding of Sp1 to GC-rich elements within the caveolin-1 promoter. ChIP assay. Chromatin immunoprecipitation assay was done on chromatin derived from untreated or hydrogen peroxide-treated (150 μM for 2 h) NIH 3 T3 cells 48 h after oxidative stress using an antibody probe specific for Sp1. PCR was performed using primers surrounding the region of the caveolin-1 promoter containing the two GC-rich boxes. Amplification of input DNA from both untreated and H2O2-treated cells was performed before immunoprecipitation. A vector containing the entire caveolin-1 promoter sequence was used as a positive control for PCR. Figure adapted from ref. (30).