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. Author manuscript; available in PMC: 2013 Jul 10.
Published in final edited form as: Autophagy. 2010 May 16;6(4):523–541. doi: 10.4161/auto.6.4.11811

Figure 1.

Figure 1

IL-12 or IFNα promote human Th1/Tc1 polarization during induction of rapamycin resistance. Lymphocytes were isolated from normal donors and co-stimulated with anti-CD3, anti-CD28 with six-day culture. In addition to co-stimulation, cytokines were added as indicated: IL-2, IL-12, IFNα and rapamycin. (A) Representative intra-cellular (IC) flow cytometry data for T cells expanded in IL-2 + IFNα + rapamycin (i–iv). On day 6 of culture, T cells were restimulated and stained with surface CD4 (i and ii), surface CD8 (iii and iv), IC isotype control (i and iii), or IC IFNγ (ii and iv). (B) Frequency of CD4+IFNγ+ cells by IC flow cytometry for the specific culture conditions (part i; n = 3 replicates per condition). IFNγ secretion after day 6 repeat co-stimulation (part ii; Multiplex suspension array on culture supernatants, n = 3 per condition). *indicates that observed differences were statistically significant (p < 0.05) as compared to IFNα groups.